The Simultaneous Determination of Some Water-Soluble Vitamins in Gum of Acacia nilotica by High Performance Liquid Chromatography
Sarra Bouazizi *
Institute of Higher and Continuing Education, Laboratory of Chemical Analysis, 43 Liberty Street, 2019 Bardo, Tunis, Tunisia
Bassem Jamoussi
Institute of Higher and Continuing Education, Laboratory of Chemical Analysis, 43 Liberty Street, 2019 Bardo, Tunis, Tunisia
Dalila Bousta
National Institute of Aromatic Medicinal Plants, BP 7048 - Ez-Kalaa, 7048, Fez, Morocco
*Author to whom correspondence should be addressed.
Abstract
A rapid, simple and precise method by HPLC (high performance liquid chromatography) has been developed for simultaneous determination of water-soluble vitamins as thiamine(B1), nicotinamide(B3), panthotenic(B5), pyridoxine(B6) and biotin(B8) in gum of Acacia nilotica using enzymatic hydrolysis. The method uses a C18 column (4.6×150 mm, 5µm). Mobile phase such as methanol 0.1M, sodium dihydrogen phosphate (pH = 2.5), (10:90 v/v) is found most suitable for rapid separation and identification of this water –soluble vitamins. Good linearity was observed between the concentration of analytes and peak area (r = 0.9999). Each vitamin was quantitatively determined at its maximum wavelength. Recovery percentages ranged from 97% to 99%.
Keywords: Water, soluble vitamins, gum, Acacia nilotica, HPLC