Recombinant Protein rP21 from Trypanosoma cruzi has Effect on Inflammation, Angiogenesis and Fibrogenesis in Skin Wound Model C57BL/6 Mouse

Jeranice Silva Barbosa

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil.

Francyelle Borges Rosa de Moura

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil. and Institute of Biology, State University of Campinas, Campinas, SP, Brazil.

Bruno Antonio Ferreira

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil and Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.

Flávia Alves Martins

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil.

Elusca Helena Muniz

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil.

José Augusto Leoncio Gomide

Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, MG, Brazil.

Cláudio Vieira da Silva

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil.

Daniele Lisboa Ribeiro

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil.

Fernanda de Assis Araújo

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil.

Tatiana Carla Tomiosso *

Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, MG, Brazil.

*Author to whom correspondence should be addressed.


Abstract

Aims: Recombinant proteins rP2 has demonstrated biological activity in inflammation by acting on the recruitment of leukocytes and by inducing phagocytosis, also modulating the processes of angiogenesis and fibrogenesis in different experimental models. In this study we evaluated the effects of the recombinant protein rP21 from Trypanosama cruzi in cutaneous wounds.

Study Design: The wounds were induced on the back of mice and treated with rP21 at 1 µg/mL and 50 µg/mL concentration, for 3 and 7 days.

Study Location and Duration: Institute of Biomedical Sciences and Animal Breeding Network and Rodents of the Federal University of Uberlândia, between February 2015 and February 2016.

Methodology: The contraction time of wound, inflammatory cell activities (neutrophils and macrophages), angiogenesis and local collagen density were evaluated.

Sample: Wound induction was performed on 64 male BALB / c mice approximately 9 weeks old.

Results: Wounds treated with rP21 showed less closure time, in addition to exhibiting greater neutrophil activity in the initial phasis, which was reduced simultaneously with the increased macrophage activity. The rP21 also performed pro-angiogenic and pro-fibrogenic activity in this study model.

Conclusion: These results demonstrate for the first time the biological potential of rP21 in accelerating skin tissue repair.

Keywords: Angiogenesis, healing, inflammation, recombinant protein, skin.


How to Cite

Barbosa, J. S., Moura, F. B. R. de, Ferreira, B. A., Martins, F. A., Muniz, E. H., Gomide, J. A. L., Silva, C. V. da, Ribeiro, D. L., Araújo, F. de A., & Tomiosso, T. C. (2021). Recombinant Protein rP21 from Trypanosoma cruzi has Effect on Inflammation, Angiogenesis and Fibrogenesis in Skin Wound Model C57BL/6 Mouse. Advances in Research, 22(1), 28–37. https://doi.org/10.9734/air/2021/v22i130285

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