Comparative Production of Cellulase from Chemical and Biological Pretreated Groundnut Husk
N. Aliyu *
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
H. Kassim
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
H. Aliyu
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
R. J. Abu
Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria
Z. Adamu
Department of Biochemistry, Federal University of Technology, Minna, Nigeria
*Author to whom correspondence should be addressed.
Abstract
Lignocellulose biomass was bioconverted with Aspergillus niger and Trichoderma viride to produce cellulase. Groundnut husk is a cheap and readily available substrate with potential for bioconversion. Cellulase production from alkali and biological pretreated groundnut husk was compared in this study. Rapid production of cellulase following FPase & CMCase derived from both organisms were observed. Enzymatic activities in all the pretreated GH increased steadily from day one and peaked at day seven. Biological pretreated GH showed significant (p<0.05) increase in total cellulase and endoglucanase yield (activity) than alkali pretreated GH.
Keywords: Cellulase, groundnut husk, endoglucanase, Aspergillus niger, Trichoderma viride