Main Article Content
Madhuca longifolia is a commercially important tree species commonly known as mahua. The livelihood of large populations of tribal people depends on collection of its flowers and seeds. Almost all the parts of Mahua are utilized in diversified uses like in industry as artificial sweetner, biodiesel, food products, in soap industry etc. In the present study, a successful attempt was made to establish in vitro cultures of Mahua from nodal segments and factors influencing in vitro morphogenesis were evaluated as propagation through seeds and cuttings encounters problems. Axillary bud break (64.44%) was successfully achieved by culturing nodal segments on Murashige and Skoogs (MS) medium supplemented with 3 mg l-1Benzyladenine (BA) in nodal explants collected during the months ofJuly-September (rainy season).Shoot multiplication with maximum number of shoots, maximum number of leaves and longest shoots was achieved on MS medium supplemented with 3 mg l-1 BA when a subculture cycle of 30 days was followed. On MS medium supplemented with 2 mg l-1 Indole-3-Butyric Acid (IBA), in vitro excised shoots were successfully rooted (55.55%) after 40 days. A two step method was employed for successful hardening of rooted plantlets. Firstly, the plantlets were transferred for one week in 1/2 strength of MS liquid medium. Then, the plantlets were transferred to root trainers containing soilrite soaked with inorganic salts of ½ strength MS medium. The hardened plantlets were acclimatized firstly in a mist chamber and then in polybags in shade house. The present study provides an effective means for in vitro shoot regeneration and plantlet formation through nodal segments of Madhuca longifolia, a commercially important tropical tree with multifarious uses.
Kulkarni P S, Sharanappa G & Ramesh M R.Mahua (Madhuca indica) as a source of bio diesel in India, International Journal of Scientific Engineering and Research. 2013;4(7):2319-2329.
Patel M, Pradhan RC, Naik SN. Physical properties of fresh mahua. International Agrophysics. 2011;25:303-306.
Kumari K, Sinha R, Krishna G, Kumar S, Singh S, Srivastav A K. Sensory and Nutritional Evaluation of Value Added Products Prepared from Mahua Flower. International Journal of Current Microbiology and Applied Sciences. 2018;7:1064-1070.
Magadum A & Sridhara S N. Performance of IC engine by using mahua oil (Madhuca indica) biodiesel, International Journal of Science and Research,2017;7 (9) :345-349.
Pradhan D, Singh RK, Bendu H, Mund R. Pyrolysis of mahua seed (Madhuca indica) –production of biofuel and its characterization. Energy Conversion and Management. 2016;108:529-538.
Puhan S, Vedaraman N, Rambrahamam BV, Nagarajan G.Mahua (Madhuca indica) seed oil: A source of renewable energy in India, Journal of Scientific and Industrial Res. 2005;64:890-896.
Prasad R. Mahua: the tree of poor. International Book Distributors, Dehradun, India; 1993.
Prakash GKS. Vegetative propagation of Jatropha, Karanj and Mahua by stem cuttings, grafting, budding and air layring. M. Sc. (Forestry thesis), Department of forestry, college of agriculture, IGKV, Raipur (CG); 2006.
Pohare CV, Choudhari SW, Mane VP, Shambharkar VB, Raut. Studies on survival percentage of softwood grafting in mahua (Madhuca indica Gmel.J.F.) by using khirni (Manilkara hexandra Roxb.) as a root stock, International Journal of Forestry and Crop Improvement. 2016;7(2):167-171.
Dunstan DI, Thorpe TA, Vasil IK. Cell Culture and Somatic Cell Genetics of Plants, 3 (Academic Press, Inc. USA); 1986.
Boulay M, Green CE.: Plant Tissue and Cell Culture (Alan R Liss Inc. New York);1987.
Rout GR, Das P. Micropropagation of Madhuca longifolia (Koenig) MacBride var. latifolia Roxb. Plant Cell Rep, 1993;12:513-516.
Singh M, Bansal YK. Regeneration of plantlets from cotyledons of Madhuca latifolia Macb. cultured in vitro, Indian Journal of Forestry. 1994;17:15-19.
Bansal YK, Chibbar T. Micropropagation of Madhuca latifolia Macb. Through nodal culture. Plant Biotech. 2000;17(1):17-20.
Jagatram M, Surendran C, Parthiban KT, Sasikumar K. Micropropagation of Madhuca latifolia Macb.Journal of Tree Science, 2002;21(1&2):41-45.
Gomez KA, Gomez AA.Statistical Procedures for Agricultural Research. (John Wiley and Sons, Inc., New York);1984.
Mishra S, Padhan S. Madhuca lonigfolia (Sapotaceae): A review of its traditional uses and nutritional properties”, International Journal of Humanities and Social Science Invention. 2013;2(5):30-36.
Boral D. Aerobiology Allergenicity and Biochemistry of Madhuca indica, Annals agricultural and environmental medicine.1999;2(6):87-90.
Erik BVW, Michael W. Medicinal plants of the world. Portland, Timber Press; 2004.
Kumaran K, Mahua (Bassia latifoliaRoxb) National Workshop on TBOs at CARI, Jhansi; 2015.
Singh IS, Srivastava AK, Singh V. Improvement of some underutilized fruits through selection. Journal of Applied Horticulture, 1999;1:34-37.
Dhillon H. Studies on growth and propagation of some tree species. Ph.D. Dissertation submitted to Panjab University, Chandigarh, India; 2007.
Singh AK, Ravishankar H, Prospects of production of quality planting material of underutilized fruits. In abstract National Conference on production of quality Seeds and Planting Material Health Management in Horticulture Crops., New Delhi; 2010.
Singh S, Singh AK. Standardization of time of softwood grafting in mahua (Bassia latifolia) and khirni (Manilkara hexandra) under semi-arid environment of western India. Indian Journal of Agriculture Sciences. 2015;85(2):166-170.
Dhavala A, Rathor TS. Micropropagation of Embelia ribes Burm f. through proliferation of adult axillary shoots, In vitro Cellular & Developmental Biology- Plant, 2010;46:180-191.
Deb CR, Deb MS, Jamir NS. Effect of different factors on in vitro axillary shoot proliferation and plant regeneration of Cinnamomum tamala Nees: A spice yielding plant. Indian Journal of Biotechnology. 2014;13:520-526.
Yadav K, Lal D, Singh N. Influence of explanting season on in vitro multiplication of Celastrus paniculatus Willd. – An endangered medicinal herb, Journal of Agricultural Technology. 2011;7(5):1355-1361.
Rani S, Rana JS. In vitro Propagation of Tylophora indica - Influence of Explanting Season, Growth Regulator Synergy, Culture Passage and Planting Substrate. Journal of American Science. 2010;6:385-392.
Yadav K, Singh N. In vitro propagation and biochemical analysis of field established Wood apple (Aegle marmelos L.). Analele Universităţii din Oradea – Fascicula Biologie. 2011;18(1):23-28.
Purohit SD, Kudka G, Sharma P, Tak K. In vitro propagation of an adult tree Wrightia tomentosathrough enhanced axillary branching. Plant Science. 1994;103:67-72.
Mao AA, Wetten A, Fay M, Caligari PDS. In vitro propagation of Clerodendrum colebrookianumWalp, a potential natural anti-hypertension medicinal plant. Plant Cell Reports. 1995;14:493-496.
Shirin F & Maravi S. Clonal propagation of an important medicinal tree Crataeva nurvalathrough enhanced axillary branching. Journal of Herbs, Spices & Medicinal Plants, 2006;12(1/2):165-174.
Chishimba WK, Lingumbwanga E, Tembo LM, Hang'andu AK. Effect of cytokinins on in vitro propagation of Uapaca kirkiana, Journal of Tropical Forest Science. 2000;12(1):28-36.
Fratini R, Ruiz ML. Comparative Study of Different Cytokinins in the Induction of Morphogenesis in Lentil (Lens culinaris Medik) In vitro Cellular & Developmental Biology-Plant, 2002;38(1):46-51
Bonga JM, Von Aderkas P. In vitro culture of trees. Kluwer Academic Publishers, Dordrecht; 1992.
Murashige T. Plant propagation through tissue culture. Annual Review of Plant Physiology, 1974;25:135-166.
Street HE. Plant tissue and cell culture, 2nd ed, London, Edinburgh, Melbourne: Blackwell Scientific Publications; 1977.
Schwarz OJ. Plant growth regulator effects in the in vitro propagation of Three Hardwood Tree Genera: Castanea, Juglans, and Quercus. Plant growth regulations, 1987;6:113-135.
Gianfagna T. Natural and synthetic growth regulators and their use in horticultural and agronomic crops.ed Davies P.J:Plant Hormones: Physiology, Biochemistry and Molecular Biology .Kluwer Academic Publishers, Dordrecht. 1995;751-773.
Gasper T, Coumans M. Root formation.ed. J.M. Bonga and D.J. Durzan: Cell and Tissue Culture in Forestry, Vol-2,Specific Principles and Methods: Growth and Developments, Martinus Nihoff Publishers, Dordrecht; 1987.
Zolman BK, Yoder A, Bartel B. Genetic analysis of indole-3-butyric acid responses in Arabidopsis thaliana reveals four mutant classes. Genetics. 2000;156:1323–1337.
Hartmann HT, Kester DE, Davies FT. Anatomical and physiological basis of propagation by cuttings. In Plant propagation: principles and practices Englewood Cliffs, NJ: Prentice Hall; 1990.