FTIR and UV-Visible Spectrophotometric Derivatization Studies of Artemether

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Jane I. Ugochukwu
Eze E. Ajaegbu
Chidinma R. Chukwunwejim
Ikemefuna C. Uzochukwu


This study was aimed at confirmation of the purity and identity of the artemether and development of an ultraviolet spectrophotometric method for the assaying of artemether utilizing p-nitroaniline as a derivatizing agent. Melting point and Fourier transform infrared spectrophotometer (FTIR) methods were used to confirm the purity and identity of the artemether used in the study. The derivatization process was carried out between 100 µg/ml of the artemether and 500 µg/ml of p-nitroaniline in different molarities (0.5, 1, 2, 3, 4, 5M) of HCl at 60ºC for 45 mins. The melting point ranged between 86-89ºC and the FTIR determination revealed a band at 3169.978 cm-1 due to O-H stretching vibration and C-H stretching at 2996.039 cm-1. There was no observed difference in wavelength between the p-nitroaniline spectrum and the spectra of the derivatized products in different molarities of HCl. p-nitroaniline may not be a proper derivatization reagent for the assay of artemether in pure or dosage form.

Artemether, p-nitroaniline, spectrophotometry, derivatization, HCL.

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How to Cite
Ugochukwu, J. I., Ajaegbu, E. E., Chukwunwejim, C. R., & Uzochukwu, I. C. (2020). FTIR and UV-Visible Spectrophotometric Derivatization Studies of Artemether. Advances in Research, 21(10), 25-31. https://doi.org/10.9734/air/2020/v21i1030248
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Burrows JN, Duparc S, Gutteridge WE, van Huijsduijnen RH, Kaszubska W, Macintyre F, Mazzuri S, Möhrle JJ, Wells TNC. New developments in anti-malarial target candidate and product profiles. Malar J. 2017;16(1):26.

Olaniyi AA. Essential Madicinal Chemistry 3rded .Hope Publication, Ibadan. 2005; 404-429.

Babalola CP, Oluwalana I, Kotila OA, Adegoke OA, Kolade YT, Ameh SJ. A novel derivatization ultraviolet spectro-photometric method for the determination of dihydroartemisinin using p-Nitroaniline. Tropical Journal of Pharmaceutical Research. 2014;13(1):127-133.

Christian J, Shah P, Patel M, Patel M, Patel K, Gandhi T. Optimizing derivatization conditions using an experimental design and Simultaneous estimation of Artemether and Lumenfantrine by ratio First order derivatives Spectrophotometric Method. J. of Taibah University. 2017;11(5):729-740.

International Pharmacopoeia. 3rd.edn. Geneva: World Health Organization, 2003; 215-222.

United States Pharmacopoeia (USP). United States Pharmacopoeia Convention, Maryland, United States. Drug Development and Industrial Pharmacy. 2016;42(10):1708–1721

Kuntworbe N, Acquah FA, Johnson R, Ofori-Kwakye K. Comparison of the physicochemical properties and in vivo bioavailability of generic and innovator artemether-lumefantrin tablets in Kumasi. Ghana Journal of Pharmacy & Pharmacognosy Research. 2018;6(3):167-178.

Pratap YP, Manisha PC, Geetanjali KG, Manish AR, Harshada PB.Validated spectrophotometric method for quantitative determination of Artemether in pharmaceutical formulation. Der Pharma Chemica. 2011;3(3):135-139.

Fule RA, Meer TS, Sav RA, Amin DP. Dissolution rate enhancement and physicochemical characterization of artemether and lumefantrin solid dispersions. Int J Drug Del. 2012;4:95-106.

Yahaya ZS, Ofokansi KC, Allagh ST, Bhatia PG. Preparation and characterization of artemether inclusion complexes with hydroxypropyl-β-cyclodextrin. Tropical Journal of Pharmaceutical Research. 2017;16(10): 2359-2364.

Joanna K. Basic principles and analytical application of derivative spectro-photometry. In. Jamal U. editor. Macro to Nano Spectroscopy. 1st ed. Croatia: In Tech. 2012;253-68.

Sowjanya G, Ganapaty S, Sara A. Derivative Uv Spectroscopic approaches in multicomponent analysis – A review. Int J Pharm Pharm Sci. 2018;11(2):1-11.

Elimam MM, Shantier SW, Gadkariem EA, Mohamed MA. Derivative Spectro-photometric methods for the analysis and stability of Colistin Sulphate. Journal of Chemistry. 2015;1-5.

Gorai S. Preferential solvation of p-nitroaniline in alcohol-dioxan binary solvent mixture. A Dissertation Submitted for the partial fulfilment for the degree of Master of Science in Chemistry, Academic Autonomy National Institute of Technology, ROURKELA – 769008, ORISSA; 2010.