Assessment of Sub Chronic Toxicity of Sonchus cornutus in Rats
Advances in Research,
Aim: To assess the toxicity of the aqueous extract of Sonchus cornutus in Wistar albino rats.
Methodology: The aqueous extract of Sonchus cornutus aerial part was administered orally to rats in group 2, 3 and 4 at a dose of 50, 500 and 2000 mg/ kg, respectively for four weeks whereas, group 1 was kept as a control. The animals were observed daily for clinical signs and mortality. Weekly, the weights of the animals were recorded, and blood samples were collected for haematological and biochemical analysis. Specimens of Liver and kidney were kept in 10% formalin for histopathology.
Results: The results revealed that all the animals in the four groups survived. The extract had no adverse effects on haematology, biochemistry and histology of rats at doses of 50 and 500 mg/ kg. But dose 2000 mg/kg proved to have significant alteration in White blood cells (WBCs), Red blood cells (RBCs), Haemoglobin (Hb) and Packed cell volume (PCV). In addition, total protein, albumin, urea, creatinine, Alanin Transaminase (ALT), Asparate Transaminase (AST) were significantly (P<0.05) changed. These findings correlated with the histopathological changes on liver and kidney.
Conclusion: The highest dose of S. cornutus aqueous extract (2000 mg/kg) was not fatal, but may have some toxic effects on liver and kidney.
- Sonchus cornutus
- aqueous extract
How to Cite
Farah HF, Elamin TH, El Hussein AM, Khalid HE. Assessment of antitheilerial activity of Kigelia africana fruits against Theileria lestoquardi. Eur J Med Plants. 2015;5(1):101-108.
Maiga A, Diallo D, Fane S, Sango K, Paulsen BS, Cisse B. A survey of toxic plants on the market in the district of Bamako, Mali: Traditional knowledge compared with literature search of modern pharmacology and toxicology. J Ethnopharm. 2005;96:183-193.
Chandra SJ, Sandhya S, Vinod KR, David B, Sudhakar K, chaitanga R, Plant toxins harmful effects. Hygeia J Drugs Med. 2012;4(1):70—90.
Braun M, Burgstaller H, Hamdoun AM, Walter H. Sonchus Cornutus, Asteraceae, common weeds of central Sudan. Vier-Türme-verlag, Benedict Press, Münster schwarzach, Germany, Weinketsheim: Mergrraph; 1991.
Khan RA, Khan MR, Sahreen S, Bokhari J. Prevention of CCl4 induced nephrotoxicity with Sonchus asper in rat. Food Chem Toxicol Suppl 8-9. 2010;48:2469- 2476.
Khan MR, Badar I, Siddiquah A. Prevention of hepato-renal toxicity with Sonchus asper in gentamicin treated rats. Complement Altern Med. 2011;11:113.
Khan RA. Protective effects of Sonchus asper (L.) Hill, (Asteraceae) against CCl4 induced oxidative stress in the thyroid tissue of rats. BMC Complement Altern Med. 2012;12:181-188.
Mustaq MN, Akhtar MS, Alamgeer, Ahmad T, Khan HU, Maheen S, Tabassum N, et al. Evaluation of antihypertensive activity of Sonchus asper L. in rats. Acta pol phar. 2016;73(2):425-431.
[PMID: 27180435] [Indexed for MEDLINE]
Farah HM, Elamin TH, Khalid HE, Elhussein AM. Evaluation of in vitro antitheilerial activity of Sonchus cornutus aqueous extract. Topcls J Herb Med. 2013; 2(5):90-94.
Han Y, Zhang G, Gav K, Jia Z. Natural product chemistry new sesquiterpens from S. transpicus. Planta Med. 2005;71:543-547.
Leonard DB. Plants and food. Sonchus oleraceus. Med at your Feet. 2007;1-4.
Fashir GA, Abdalla NI, Fangama IM. Assessment the consumption of Sonchus cornutus (Hochst) in Khartoum State, Sudan. Intern J Curr Microbiol Appl Sci. 2015;4(6):833-839.
Harborne JB. editor. Phytochemical methods. 2nd ed, Chapman and Hall Ltd., London. 1984;4-7.
Wegiera M, Smolarz HD, Jeduch M, korczak M, Kopron K. Cytotoxic effect of some medicinal plants from Asteraceae family on J-45.01 leukemic cell lines. Pilot study. Acta Pol pharm. Drug Res. 69(2): 263-268.
Nyarko AK, Okine LKN, Wedzi RK, Addo PA, Ofosuhne M. Sub chronic toxicity of the antidiabetic herbal preparation ADD 199 in the rat: Absence of organ toxicity and modulation of cytochrome P450. J Ethnopharm. 2015;97(2):319-325.
Keinänen M, Kunutila S, Bloomfield CD, Elonen E, de la Chapelle A. The proportion of mitosis in different cell line changes during short-term culture of normal human bone marrow. Blood. 1986;67:1243-1247.
Selmanoglu G, Barlas N, Songür S, Kockaya EA. Carbendazim induced haematological, biochemical and histopathological changes to the liver and kidney of male rats. Hum Exp Toxicol. 2001;20:625-630.
Cheesbrough M. Medical laboratory manual for tropical countries. Microbiology Tropical Health Technology/ Butterworth Scientific Publications, Boston; 1991.
Wuruchekke AU, Anthony AG, Obiolah W. Biochemical effects on liver and kidney of rats administered aqueous extract of Xemenia Americana. African J Biotech. 2008;7:2777-2780.
Sharma A, Hirluukar NB, Wadel P, Das P. Influence of hyperglycemia on renal function parameter in patients with Diabetus mellitus. Int J Pharmaceut Biol Arch. 2011;2:734-739.
Bayramoglu G, Senturk H, Bayramonglu A, Uyanoglu M, Colka S, Ozmen A, et al. Carvacrol partially reserves symptoms of diabetes in STZ- induced diabetic rats. Cytotech. 2014;66:215-217.
Madinov IV, Balabadkin MI, Markov DS, Markov TN, Main causes of hyperureamia in diabetes mellitus. Terk Arkh. 2000;55-58.
Nurianti Y, Henandriani R, Sukandar EY, Anggadiredja K. Acute and subchronic oral toxicity studies of ethylacetate extract of Sonchus arvensis L. leaves. Int J Pharm and Pharmaceut Sci. 2014;65(5):343-347.
Abstract View: 624 times
PDF Download: 223 times