Selection of Genotype/Mutant through Mutation Breeding Approach for Establishment of Mung Bean (Vigna radiata (L.) Wilczek) Tilling Population
Anusheela Varadaraju *
Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
Bharathi Raja Ramadoss
Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
John Joel
Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
Kulandaivelu Ganesamurthy
Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
Sundaram Ganesh Ram
Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
*Author to whom correspondence should be addressed.
Abstract
The present study investigated the effects of EMS on crop phenotypes on four mungbean varieties viz., Co (6), Co (7), VRM and VBM. The germination percentage was affected by EMS treatment with increase in dose or concentration of mutagen. The doses can be determined by establishing the LD 50 value for the mutagen to be used. The expected LD50 values of EMS for Co (6), Co (7), VRM 1 and VBM 3 are 37.15, 39.81, 28.18, 32.35 respectively. Several plant traits like germination percentage, survival percentage, root length, shoot length, was affected with the mutagenic treatments. Co7 shows better germination and survival percentage compared to other genotypes. Hence Co7 chosen for establishment of TILLING population.
Keywords: EMS, mung bean, mutagen, probit analysis, TILLING