The Role of Bio-fortification in Enhancing the Nutritional Quality of Vegetables: A Review

Badri Lal Nagar

Department of Vegetable Science, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya Gwalior (M.P.) 474002, India.

Satendra Singh Thakur

M.P. High Court, Jabalpur-482001, India.

Pawan Kumar Goutam

Department of Crop Physiology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002 (U.P.) India.

Pramod Kumar Prajapati

College of Agriculture, Tikamgarh- JNKVV, Jabalpur-482004, India.

Rakesh Kumar

Department of Genetics and Plant Breeding, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002 (U.P.), India.

Sandeep Kumar

Department of Genetics and Plant Breeding, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002 (U.P.), India.

Rajat Singh

Department of Vegetable Science, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002 (U.P.), India.

Arun Kumar *

Department of Entomology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur-208002 (U.P.), India.

*Author to whom correspondence should be addressed.


Abstract

Bio-fortification is a process that enhances the nutritional quality of crops, including vegetables, by increasing their micronutrient content. It can be achieved through different approaches such as agronomic, conventional breeding, and transgenic/biotechnological methods. Vegetables are known to be rich in micronutrients, vitamins, antioxidants, and other health-benefiting compounds, making them essential for a balanced diet. However, malnutrition and hidden hunger continue to be global challenges, particularly in developing countries. Micronutrient deficiencies, such as iron, zinc, iodine, and vitamin A, are prevalent in these populations. Conventional breeding focuses on selecting genotypes with desirable nutritional traits without compromising agricultural productivity. Transgenic/biotechnological approaches involve the synthesis of transgenes to enhance the bioavailability of micronutrients in plants. Bio-fortification of vegetables is particularly important as they are rich sources of micronutrients, vitamins, and other health-benefiting compounds. However, improving the nutritional quality of vegetables through conventional breeding has had limited success, and modern molecular tools and techniques offer potential for handling complex traits and developing nutrient-dense varieties. Bio-fortification offers a sustainable solution to address these deficiencies by increasing the nutrient content of crops, particularly plant-based foods. These techniques have shown promising results in increasing the concentration of nutrients, such as iron, in vegetables, thereby improving their nutritional quality.

Keywords: Bio-fortification, biotechnological methods, malnutrition, micronutrients and vitamins


How to Cite

Nagar, B. L., Thakur, S. S., Goutam, P. K., Prajapati, P. K., Kumar, R., Kumar, S., Singh, R., & Kumar, A. (2024). The Role of Bio-fortification in Enhancing the Nutritional Quality of Vegetables: A Review. Advances in Research, 25(4), 64–77. https://doi.org/10.9734/air/2024/v25i41082

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References

Bouis HE, Welch RM. Biofortification—a sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop Science. 2010;50:S-20.

Ofori KF, Antoniello S, English MM, Aryee AN. Improving nutrition through biofortification–A systematic review. Frontiers in Nutrition. 2022;9: 1043655.

Singh SS, Hazra KK, Praharaj CS, Singh U. Biofortification: Pathway ahead and future challenges. Bio Fortification of Food Crops. 2016;479-492.

Msungu SD, Mushongi AA, Venkataramana PB, Mbega ER. A review on the trends of maize biofortification in alleviating hidden hunger in sub-Sahara Africa. Scientia Horticulturae. 2022;299: 111029.

Koç E, Karayiğit B. Assessment of biofortification approaches used to improve micronutrient-dense plants that are a sustainable solution to combat hidden hunger. Journal of Soil Science and Plant Nutrition. 2022;22(1):475-500.

Liu RH. Health-promoting components of fruits and vegetables in the diet. Advances in Nutrition. 2013;4(3):384S-392S.

Avnee, Sood S, Chaudhary DR, Jhorar P, Rana RS. Biofortification: An approach to eradicate micronutrient deficiency. Frontiers in Nutrition. 2023;10: 1233070.

Kumar S, Palve A, Joshi C, Srivastava RK. Crop biofortification for Iron (Fe), zinc (Zn) and vitamin A with transgenic approaches. Heliyon. 2019;5(6).

Bhardwaj AK, Chejara S, Malik K, Kumar R, Kumar A, Yadav RK. Agronomic biofortification of food crops: An emerging opportunity for global food and nutritional security. Frontiers in Plant Science. 2022;13:1055278.

Ashoka P, Spandana B, Saikanth DRK, Kesarwani A, Nain M, Pandey SK, Maurya CL. Bio-fortification and its impact on global health. Journal of Experimental Agriculture International. 2023;45(10):106-115.

Sheoran S, Kumar S, Ramtekey V, Kar P, Meena RS, Jangir CK. Current status and potential of biofortification to enhance crop nutritional quality: An overview. Sustainability. 2022;14(6):3301.

Wakeel A, Farooq M, Bashir K, Ozturk L. Micronutrient malnutrition and biofortification: Recent advances and future perspectives. Plant Micronutrient Use Efficiency. 2018;225-243.

Prasad BVG, Mohanta S, Rahaman S, Bareily P. Bio-fortification in horticultural crops. Journal of Agricultural Engineering and Food Technology. 2015;2(2):95-99.

Garcia‐Casal MN, Peña‐Rosas JP, Giyose B. Consultation Working Groups. Staple crops biofortified with increased vitamins and minerals: Considerations for a public health strategy. Annals of the New York Academy of Sciences. 2017;1390(1):3-13.

Darmon N, Darmon M, Maillot M, Drewnowski A. A nutrient density standard for vegetables and fruits: Nutrients per calorie and nutrients per unit cost. Journal of the American Dietetic Association. 2005;105(12):1881-1887.

Hoque M, Emon K, Malo PC, Hossain MH, Tannu SI, Roshed MM. Comprehensive guide to vitamin and mineral sources with their requirements. Indiana Journal of Agriculture and Life Sciences. 2023;3(6): 23-31.

Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. International Journal of Biomedical Science: IJBS. 2008;4(2):89.

Ötles S, Ozgoz S. Health effects of dietary fiber. Acta scientiarum polonorum Technologia alimentaria. 2014;13(2):191-202.

Butt MS, Sultan MT. Nutritional profile of vegetables and its significance in human health. Handbook of Vegetables and Vegetable processing. 2018;157-180.

Rolls BJ, Drewnowski A, Ledikwe JH. Changing the energy density of the diet as a strategy for weight management. Journal of the American Dietetic Association. 2005;105(5):98-103.

Boeing H, Bechthold A, Bub A, Ellinger S, Haller D, Kroke A, Watzl B. Critical review: Vegetables and fruit in the prevention of chronic diseases. European Journal of Nutrition. 2012;51:637-663.

Lordan C, Thapa D, Ross RP, Cotter PD. Potential for enriching next-generation health-promoting gut bacteria through prebiotics and other dietary components. Gut Microbes. 2020;11(1):1-20.

Abdel-Aal ESM, Akhtar H, Zaheer K, Ali R. Dietary sources of lutein and zeaxanthin carotenoids and their role in eye health. Nutrients. 2013;5(4):1169-1185.

McGrattan AM, McGuinness B, McKinley MC, Kee F, Passmore P, Woodside JV, McEvoy CT. Diet and inflammation in cognitive ageing and Alzheimer’s disease. Current Nutrition Reports. 2019;8:53-65.

Dhok A, Butola LK, Anjankar A, Shinde ADR, Kute PK, Jha RK. Role of vitamins and minerals in improving immunity during Covid-19 pandemic-A review. Journal of Evolution of Medical and Dental Sciences. 2020;9(32):2296-2301.

Lucius K. Botanical Medicine and Phytochemicals in Healthy Aging and Longevity—Part 1. Alternative and Complementary Therapies. 2020;26(1):31-37.

Vassilakou T. Childhood malnutrition: Time for action. Children. 2021;8(2):103.

Erokhin V, Diao L, Gao T, Andrei JV, Ivolga A, Zong Y. The supply of calories, proteins, and fats in low-income countries: A four-decade retrospective study. International Journal of Environmental Research and Public Health. 2021;18(14):7356.

Singh A, Karmakar S, Jacob BS, Bhattacharya P, Kumar SJ, Banerjee R. Enzymatic polishing of cereal grains for improved nutrient retainment. Journal of Food Science and Technology. 2015;52:3147-3157.

Comerford KB, Miller GD, Reinhardt Kapsak W, Brown KA. The complementary roles for plant-source and animal-source foods in sustainable healthy diets. Nutrients. 2021;13(10):3469.

Lalani B, Bechoff A, Bennett B. Which choice of delivery model (s) works best to deliver fortified foods? Nutrients. 2019;11 (7):1594.

Kumari M, Sharma D, Sandeep S. Bio fortification of vegetable crops: An option for mitigating hidden hunger. International Journal of Economic Plants. 2022;9(3): 184-193.

Bhardwaj AK, Chejara S, Malik K, Kumar R, Kumar A, Yadav RK. Agronomic biofortification of food crops: An emerging opportunity for global food and nutritional security. Frontiers in Plant Science. 2022;13:1055278.

Douthwaite B. Mainstreaming of biofortification in the African Union: Evaluation of CGIAR contributions to a policy outcome trajectory; 2020.

Wakeel A, Arif S, Bashir MA, Ahmad Z, Rehman HU, Kiran A, Khan MR. Perspectives of folate biofortification of cereal grains. Journal of Plant Nutrition. 2018;41(19):2507-2524.

Debelo H, Novotny JA, Ferruzzi MG. Vitamin a. Advances in Nutrition. 2017;8(6):992.

Naidu KA. Vitamin C in human health and disease is still a mystery? An overview. Nutrition Journal. 2003;2:1-10.

Klack K, De Carvalho JF. Vitamin k: Metabolism, sources and interaction with foods and oral anticoagulants. International Journal of Medical and Biological Frontiers. 2011;17(4/5):351.

Hrubša M, Siatka T, Nejmanová I, Vopršalová M, Kujovská Krčmová L, Matoušová K, Oemonom. Biological properties of vitamins of the B-complex, part 1: Vitamins B1, B2, B3, and B5. Nutrients. 2022;14(3):484.

Palmer BF, Colbert G, Clegg DJ. Potassium homeostasis, chronic kidney disease, and the plant-enriched diets. Kidney360. 2020;1(1):65-71.

Blaszczyk U, Duda-Chodak A. Magnesium: Its role in nutrition and carcinogenesis. Roczniki Państwowego Zakładu Higieny. 2013;64(3).

Ross AC, Taylor CL, Yaktine AL, Del Valle HB. Overview of calcium. In Dietary reference intakes for calcium and Vitamin D. National Academies Press (US); 2011.

Stephen J, Manoharan D, Radhakrishnan M. Immune boosting functional components of natural foods and its health benefits. Food Production, Processing and Nutrition. 2023;5(1):61.

Bakan E, Akbulut ZT, İnanç AL. Carotenoids in foods and their effects on human health. Akademik Gıda. 2014;12(2):61-68.

Yildiz F, Kotzekidou P, Michaelidou A, Nocella G. Functional foods in Mediterranean and middle eastern countries: History, scope and dietary habits. Nutraceutical Science and Technology. 2007;6:177.

Lima GPP, Vianello F, Corrêa CR, Campos RADS, Borguini MG. Polyphenols in fruits and vegetables and its effect on human health. Food and Nutrition Sciences. 2014;1065-1082.

Poiroux-Gonord F, Bidel LP, Fanciullino AL, Gautier H, Lauri-Lopez F, Urban L. Health benefits of vitamins and secondary metabolites of fruits and vegetables and prospects to increase their concentrations by agronomic approaches. Journal of Agricultural and Food Chemistry. 2010;58(23):12065-12082.

Rouphael Y, Cardarelli M, Bassal A, Leonardi C, Giuffrida F, Colla G. Vegetable quality as affected by genetic, agronomic and environmental factors. J. Food Agric. Environ. 2012;10(3):680-688.

Jones RB, Stefanelli D, Tomkins RB. Pre-harvest and post-harvest factors affecting ascorbic acid and carotenoid content in fruits and vegetables. In XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014). 2014;1106: 31-42.

Beebe S. Biofortification of common bean for higher iron concentration. Frontiers in Sustainable Food Systems. 2020;4: 573449.

Tsakirpaloglou N, Mallikarjuna Swamy BP, Acuin C, Slamet-Loedin IH. Biofortified Zn and Fe rice: Potential contribution for dietary mineral and human health. Nutritional Quality Improvement in Plants. 2019;1-24.

Nestel P, Bouis HE, Meenakshi JV, Pfeiffer W. Biofortification of staple food crops. The Journal of Nutrition. 2006;136(4):1064-1067.

Parada J, Aguilera JM. Food microstructure affects the bioavailability of several nutrients. Journal of Food Science. 2007;72(2):R21-R32.

Jaiswal DK, Krishna R, Chouhan GK, De Araujo Pereira AP, Ade AB, Prakash S, Verma JP. Bio-fortification of minerals in crops: Current scenario and future prospects for sustainable agriculture and human health. Plant Growth Regulation. 2022;98(1):5-22.

Avnee, Sood S, Chaudhary DR, Jhorar P, Rana RS. Biofortification: An approach to eradicate micronutrient deficiency. Frontiers in Nutrition. 2023;10: 1233070.

Gastélum-Estrada A, Serna-Saldívar SO, Jacobo-Velázquez DA. Fighting the COVID-19 pandemic through biofortification: Innovative approaches to improve the immunomodulating capacity of foods. ACS Food Science and Technology. 2021;1(4):480-486.

Bommer C, Mittal N, Vollmer S. The impact of nutritional interventions on child health and cognitive development. Annual Review of Resource Economics. 2020;12:345-366.

Behera TK, Singh S. Advances in vegetable breeding for nutraceuticals and quality traits. Indian Journal of Genetics and Plant Breeding. 2019;79(Sup-01):216-226.

Ebert AW. The role of vegetable genetic resources in nutrition security and vegetable breeding. Plants. 2020;9(6):736.

Devi EL, Devi CP, Kumar S, Sharma SK, Beemrote A, Chongtham SK, Ngachan SV. Marker assisted selection (MAS) towards generating stress tolerant crop plants. Plant Gene. 2017;11:205-218.

Zhu C, Naqvi S, Gomez-Galera S, Pelacho AM, Capell T, Christou P. Transgenic strategies for the nutritional enhancement of plants. Trends in Plant Science. 2007;12(12):548-555.

Chakrabarti S, Chatterjee C, Mandal A. Improving nutrient value of crops: Applications of RNAi in targeting plant metabolic pathways. RNA-Based Technologies for Functional Genomics in Plants. 2021;199-225.

Wan L, Wang Z, Tang M, Hong D, Sun Y, Ren J, Zeng H. CRISPR-Cas9 gene editing for fruit and vegetable crops: Strategies and prospects. Horticulturae. 2021;7(7):193.

Dhaliwal SS, Naresh RK, Mandal A, Singh R, Dhaliwal MK. Dynamics and transformations of micronutrients in agricultural soils as influenced by organic matter build-up: A review. Environmental and Sustainability Indicators. 2019;1: 100007.

Alshaal T, El-Ramady H. Foliar application: From plant nutrition to biofortification. Environment, Biodiversity and Soil Security. 2017;1(2017):71-83.

Dorais M, Alsanius BW, Voogt W, Pepin S, Tuzel H, Tuzel Y, Möller K. Impact of water quality and irrigation management on organic greenhouse horticulture. Bio Greenhouse; 2016.

Nakano Y, Kobayashi Y. Genome-wide association studies of agronomic traits consisting of field-and molecular-based phenotypes. Reviews in Agricultural Science. 2020;8:28-45.

Salinier J, Lefebvre V, Besombes D, Burck H, Causse M, Daunay MC, Stevens R. The INRAE Centre for Vegetable Germplasm: Geographically and phenotypically diverse collections and their use in genetics and plant breeding. Plants. 2022;11 (3):347.

Gaikwad KB, Rani S, Kumar M, Gupta V, Babu PH, Bainsla NK, Yadav R. Enhancing the nutritional quality of major food crops through conventional and genomics-assisted breeding. Frontiers in Nutrition. 2020;7:533453.

El-Ramady HR, Domokos-Szabolcsy É, Abdalla NA, Taha HS, Fári M. Postharvest management of fruits and vegetables storage. Sustainable Agriculture Reviews: Volume. 2015;15:65-152.

De Ritter E. Effect of processing on nutrient content of food: Vitamins. In Handbook of Nutritive Value of Processed Food. CRC Press. 2019;473-510

Prasad BVG, Mohanta S, Rahaman S, Bareily P. Bio-fortification in horticultural crops. Journal of Agricultural Engineering and Food Technology. 2015;2(2):95-99.

Thakur V, Sharma A, Sharma P, Kumar P, Shilpa. Bio fortification of vegetable crops for vitamins, mineral and other quality traits. The Journal of Horticultural Science and Biotechnology. 2022;97(4): 417-428.

Darshan SN, Suneetha C, Manjunathaswamy TS, TR K, TR S, PN K. Biofortification of vegetable crops–A Review. The Pharma Journal. 2022;11 (1):1-8.

Wamiq M, Alam K, Ahmad K, Luthra S. Biofortification in Vegetable Crops. Modern Concept in. 2022;143.

Dhanasekar P, Souframanien J, Suprasanna P. Breeding cowpea for quality traits: A genetic biofortification perspective. Breeding for enhanced nutrition and bio-active compounds in food legumes. 2021;157-179.

Baligar VC, Fageria NK, He ZL. Nutrient use efficiency in plants. Communications in Soil Science and Plant Analysis. 2001;32(7-8):921-950.

Husaini AM. High-value pleiotropic genes for developing multiple stress-tolerant biofortified crops for 21st-century challenges. Heredity. 2022;128(6):460-472.

Samuel L, De Barcellos MD, Watabaji MD, De Steur H. Factors affecting farmers’ acceptance and adoption of biofortified crops: A systematic review. Outlook on Agriculture. 2024;53(1):15-29.

Talsma EF, Melse-Boonstra A, Brouwer ID. Acceptance and adoption of biofortified crops in low-and middle-income countries: A systematic review. Nutrition Reviews. 2017;75(10):798-829.

Mitra‐Ganguli T, Pfeiffer WH, Walton J. The global regulatory framework for the commercialization of nutrient enriched bio fortified foods. Annals of the New York Academy of Sciences. 2022;1517(1):154-166.

Bouis H, Birol E, Boy E, Gannon BM, Haas JD, Low J, Welch RM. Food biofortification: Reaping the benefits of science to overcome hidden hunger. In October webinar on The Need for Agricultural Innovation to Sustainably Feed the World by 2050. Council for Agricultural Science and Technology (CAST). 2020;69.