Preparation, Characterization, and Biological Assessment of Heterocyclic Schiff Base Metal Complexes

Y. Prashanthi *

Department of Chemistry, Mahatma Gandhi University, Nalgonda–508254,India.

K. Kiranmai

Department of Chemistry, Andhra Mahila Sabha Arts and Science College for Women, Hyderabad-500 007, India.

Tentu Nageswara Rao

Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India.


Department of Chemistry, Osmania University, Hyderabad-500 007, India.

*Author to whom correspondence should be addressed.


MIIMC, MMIIMC, and CMIIMC are biologically significant Schiff bases formed by the condensation of 3-amino-5-methyl isoxazole with formyl chromone. Substituted formyl chromones and metal chelates containing Cu(II), Ni(II), Co(II), and Zn(II) are prepared. These were studied utilising elemental analysis, FTIR, UV-VIS, 1H, 13C, mass spectra, magnetic susceptibility, conductance tests, and thermogravimetric techniques. The data revealed that the ligands display neutral, bidentate coordination through carbonyl oxygen and azomethine nitrogen. The antibacterial activity of the ligands and metal complexes is determined in vitro against bacteria and fungus using the paper disc method. Metal chelates have significantly higher MIC values against bacteria than ligands. The DNA binding properties of Cu(II) complexes were examined. MTT is used to determine the complexes' anticancer efficacy against HL60 tumour cells. The researchers discovered that the complexes prevented apparent morphological alterations. The inhibition ratio was hastened by increasing the dosage, and it had a strong positive association with the medicine dosage.

Thermal analysis of complexes showed initial mass loss due to moisture and hydrated water molecules, followed by weight loss at 220oC due to coordinated water molecules, indicating metal oxide breakdown.

Keywords: Biological assessment, heterocyclic schiff base metal complexes, FTIR, UV-VIS, 1H, 13C, mass spectra

How to Cite

Prashanthi , Y., Kiranmai , K., Rao , T. N., & , S. (2024). Preparation, Characterization, and Biological Assessment of Heterocyclic Schiff Base Metal Complexes. Advances in Research, 25(4), 47–56.


Download data is not yet available.


Marciniak B, Buono-Core GE. Photochemical properties of 1,3-diketonate transition metal chelates. j. photochem. photobiol. A Chem. 1990;52:1–25, DOI: 10.1016/1010-6030(90)87085-P

Chohan ZH, Iqbal MS, Iqbal HS, Scozzafava A, Supuran CT. Transition metal acetylsalicylates and their anti-inflammatory activity. J. Enzyme Inhib. Med. Chem. 2002;17:87–91, DOI: 10.1080/14756360290030734

López Tévez LL, Martínez Medin JJ, Islas MS, Piro OE, Castellano EE, Bruzzone L, Ferrer EG, Williams PAM. Antibacterial activity assays of a new cadmium complex with o-phenanthroline and cyanoguanidine: Crystal structure,fluorescence properties, and chemical speciation studies. J. Coord. Chem. 2011;64:3560–3574, DOI: 10.1080/00958972.2011.626854

El Fannassi Y, Gharsallaoui A, Khelissa S, El Amrani MA, Suisse I, Sauthier M, Jama C, Boudra S, Chihib NE. Complexation of terpenes for the production of new antimicrobial and antibiofilm molecules and their encapsulation in order to improve their activities. Appl. Sci. 2023;13. DOI: 10.3390/app13179854.

GUO SB. Ournal Of. Antimicrobial Activity of Copper(II) and Cobalt(II) Complexes of Citral-Valine Derived Schiff Base Asian J. Chem. 2014;26:70–73.

Uivarosi, V. Metal complexes of quinolone antibiotics and their applications: An update. Molecules. 2013;18:11153–11197, DOI: 10.3390/molecules180911153

Hu J, Sun J, Zhou T, Xu Y. Microwave-assisted synthesis of 1,3,4-thiadiazole schiff base derivatives. J. Chem. Res. 2011;35:442–443, DOI:10.3184/174751911X13128202305728

Abu-Dief AM, Mohamed IMA. A review on versatile applications of transition metal complexes incorporating schiff bases. Beni-Suef Univ. J. Basic Appl. Sci. 2015;4:119–133 DOI: 10.1016/j.bjbas.2015.05.004

Kalanithi M, Rajarajan M, Tharmaraj P. Spectral, biological screening, and DNA studies of metal chelates of 4-[N,N-Bis-(3,5-Dimethyl-Pyrazolyl-1-Methyl)]Aminoantipyrine. J. Coord. Chem. 2011;64:1436–1445, DOI: 10.1080/00958972.2011.572965

Sadeek SA, Mohamed AA, El-Sayed HA, El-Attar MS. Spectroscopic characterization, thermogravimetric and antimicrobial studies of some new metal complexes derived From4-(4-Isopropyl Phenyl)-2-Oxo-6-Phenyl 1,2-Dihyropyridine-3-Carbonitrile (L). Appl. Organomet. Chem. 2020;34:2–17.

DOI: 10.1002/aoc.5334

Abdel A, El MS, Abdelhamid AO. European Journal of Chemistry. 2012; 3:455–460 DOI:10.5155/eurjchem.3.3.359

Bajroliya S, Kalwania GS, Choudhary S, Chomal S. Synthesis, characterization and antimicrobial activities of 1,2,4-Triazole /Isatin Schiff Bases and Their Mn(II), Co(II) Complexes. Orient. J. Chem. 2014;30: 1601–1608. DOI: 10.13005/ojc/300419

Anupama B, Padmaja M, Kumari CG. Synthesis, Characterization, biological activity and DNA binding studies of metal complexes with 4-aminoantipyrine schiff base ligand. E-Journal Chem. 2012;9:389–400.

DOI: 10.1155/2012/291850

El-Tabl AS, Wahed MMA El, Mohamed EA, Abu-Setta MHH. Modulation of cancer therapy using nano-organometallic compounds: Preparation, spectroscopic characterization and cytotoxic evaluation. Egypt. J. Chem. 2021;64:3873–3887. DOI: 10.21608/ejchem.2021.64313.3379

El-tabl AS, El-wahed MMA, El-henawy EM, El-wahab OEA, Mohamed S, Hashim F. Metal complexes in cancer therapy: synthesis, spectroscopic characterization and antitumor effect against breast cancer of new dihydroxy phenyl ethylidene amino benzoic acid complexes. 2019;10: 631–645.

Vijayalakshmi M. Synthesis and spectral characterization of schiff base transition metal complexes, DNA cleavage and antibacterial activity studies. Rasayan J. Chem. 2018;11:857–864, DOI: 10.7324/RJC.2018.1123033

Lamani D, Reddy K, Naik H. An efficient synthesis and DNA binding interaction study of some novel heterocyclic fused-pyarzolequinolines: A potent antimicrobial agent. African J. Pure Appl. Chem. 2010;4:247–255.

Prashanthi Y, Kiranmai K, Subhashini NJP. Shivaraj synthesis, potentiometric and antimicrobial studies on metal complexes of isoxazole schiff bases. Spectrochim. Acta - Part A Mol. Biomol. Spectrosc. 2008;70:30–35, DOI: 10.1016/j.saa.2007.07.02

Kumar SV, Kumar SP, Rupesh D, Nitin K. Journal of Chemical and Pharmaceutical Research Preparations. 2011;3: 675–684.

Prashanthi Y, Raj S. Synthesis and characterization of transition metal complexes with. J. Sci. Res. 2009;2: 114–126. DOI:10.3329/jsr.v2i1.2732