Studies on the Persistence of Pyraclostrobin 10% CS in Acidic, Neutral and Basic Waters by Validated HPLC-DAD Method

Joga Simhachalam

Department of Engineering Chemistry, AUCE (A), Andhra University, Visakhapatnam-530003, Andhra Pradesh, India.

Gandham Hima Bindu *

Department of Engineering Chemistry, AUCE (A), Andhra University, Visakhapatnam-530003, Andhra Pradesh, India.

*Author to whom correspondence should be addressed.


This paper presents a method for the determination of pyraclostrobin 10% CS, a fungicide used in agriculture, in water samples with different pH values. The method is based on high-performance liquid chromatography with diode array detection (HPLC-DAD) and uses a reversed-phase column and a gradient elution. The method was validated according to the SANCO guidelines and showed good linearity, accuracy, precision, sensitivity and selectivity. The method was applied to study the persistence of pyraclostrobin 10% CS in acidic, neutral and basic water under laboratory conditions. The results showed that pyraclostrobin 10% CS was degraded rapidly in basic water with a half-life of less than ten days. The degradation products were identified by HPLC. The study involved exposing water samples to direct sunlight until the end of the experiment. The water samples had different pH levels: acidic (4.0), neutral (7.0) and basic (9.0). The water samples also contained Pyraclostrobin, a fungicide. The researchers collected aliquots of the water samples at various time intervals: 0, 1, 5, 10, 15, 20 and 30 days. The analysis continued until the Pyraclostrobin residues were below the detection limit. The DT50 can vary significantly depending on environmental conditions, such as pH levels.

At pH 4, pH 7, and pH 9, the reported DT50 ranges between 6 to 8.1 days, indicating a moderate rate of degradation or dissipation in these conditions. These values suggest that the substance is relatively stable across a range of acidic to basic conditions, with only slight variations in the degradation rate.

Keywords: Pyraclostrobin 10% CS, HPLC-DAD, SANCO, method validation, acidic water, basic water, neutral water

How to Cite

Simhachalam, J., & Bindu, G. H. (2024). Studies on the Persistence of Pyraclostrobin 10% CS in Acidic, Neutral and Basic Waters by Validated HPLC-DAD Method. Advances in Research, 25(4), 30–39.


Download data is not yet available.


Yang M, Zhang J, Zhang J, Rashid M, Zhong G, Liu J, The control effect of fungicide pyraclostrobin against freckle disease of banana and its residue dynamics under field conditions, J. Environ. Sci. Heal. - Part B Pestic. Food Contam. Agric. Wastes. 2018;53:615–621. Available:

Dominguez AN, Emmert GE, Gil DM, Álvarez RMS, Experimental and theoretical vibrational study of the fungicide pyraclostrobin, Spectrochim. Acta - Part A Mol. Biomol. Spectrosc. 2021;259. Available:

Joshi J, Sharma S, Guruprasad KN, Foliar application of pyraclostrobin fungicide enhances the growth, rhizobial-nodule formation and nitrogenase activity in soybean (var. JS-335), Pestic. Biochem. Physiol. 2014;114:61–66. Available:

Avenot H, Morgan DP, Michailides TJ, Resistance to pyraclostrobin, boscalid and multiple resistance to Pristine® (pyraclostrobin + boscalid) fungicide in Alternaria alternata causing alternaria late blight of pistachios in California, Plant Pathol. 2008;57:135–140. Available:

Yan Z, Cai G, Jin Z, Fu Y, Ma J, Li M, Han W, Wu Y, Determination of pyraclostrobin residue in wax gourd and its dietary risk assessment, Food Sci. Technol. 2023;43:1–6. Available:

Li H, Yang S, Li T, Li X, Huang X, Gao Y, Li B, Lin J, Mu W, Determination of pyraclostrobin dynamic residual distribution in tilapia tissues by UPLC-MS/MS under acute toxicity conditions, Ecotoxicol. Environ. Saf. 2020;206: 111182. Available:

Fulcher JM, Wayment DG, White PM, Webber CL, Pyraclostrobin wash-off from sugarcane leaves and aerobic dissipation in agricultural soil, J. Agric. Food Chem. 2014;62:2141–2146. Available:

Shen H, Yuan H, Liang J, Zhou X, Ge P, Liu Y, Gao T, Yang K, Yang D, Degradation of pyraclostrobin in water using a novel hybrid gas–liquid phase discharge reactor, Water (Switzerland). 2023;15. Available:

Han L, Wu Q, Wu X, Dissipation and residues of pyraclostrobin in Rosa roxburghii and soil under filed conditions, Foods. 2022;11:1–11. Available:

Parlakidis P, Adamidis G, Alexoudis C, Pythoglou P, Papadopoulos S, Vryzas Z, Adjuvant Effects on Pyraclostrobin and Boscalid Residues, Systemic Movement, and Dietary Risk in garlic under field conditions, Agric. 2023;13. Available:

Sad N, Dissipation rate of boscalid and pyraclostrobin fungicide in. 2020;509–514.

Patel DK, Swami BL, Rajawat TL, Development and validation of stability indicating ultra Performance Liquid Chromatography method for simultaneous Quantification of Thiophante-methyl, fipronil and pyraclostrobin in pesticide formulation, Int. J. Sci. Res. Sci. Technol. 2018;5:1308–1317.

Alam Mir W. Rao, Tentu Nageswara. Prashanthi, Yarasani. Sridhar, V. Application of silica nanoparticles in the determination of herbicides in environmental water samples using liquid chromatography-mass spectroscopy, Current Nanoscience. 2020;16: 748-756.

Tentu Nageswara Rao, Mani Panagal, Botsa Parvatamma, Nanoremediation of dimethomorph in water samples using magnesium aluminate nanoparticles, Environmental Technology & Innovation. 2020;20:101176.