Enhanced Biogas Production from Anaerobic Codigestion of Lignocellulosic Waste for Efficient Bioenergy Utilization in Heating and Combustion Engine

Main Article Content

Uduak U. Ndubuisi-Nnaji
Utibe A. Ofon
Ata O. Inyang-Enin
Georgina N. Ananso


Anaerobic digestion (AD) of lignocellulosic agro-waste such as coconut husk fibre, pineapple floret and banana stem was studied using standard protocols. A combination of physical, chemical and biological Pre-treatment was performed to facilitate the anaerobic digestion process. The experiment was carried out using laboratory-scale batch bioreactors maintained at 44.5 ± 2ºC and retention time of 25 days. Biogas production, pH, total and volatile solids concentrations (TS, VS) were also measured. With a general increase in pH after Pre-treatment of all substrates, biogas yield was significantly improved by 83.1% in all codigestion assays when compared to control (untreated substrates) with highest TS and VS removal rates of 77.7% and 87.2% respectively. In terms process performance, Pre-treatment of single substrate did not significantly improve AD of single substrates (coconut husk fibre/pineapple floret) and biogas production was inhibited in pre-treated banana stem. However, the highest biogas production with corresponding TS and VS removal rates of 78.3% and 92.9% respectively were obtained from untreated banana stem. Codigestion significantly enhanced biogas production that can be utilized for heating and knowledge of appropriate Pre-treatment choice is recommended to improve bioenergy production efficiency during anaerobic digestion.

Lignocellulosic agro-waste, anaerobic digestion, Pre-treatment; biogas.

Article Details

How to Cite
Ndubuisi-Nnaji, U. U., Ofon, U. A., Inyang-Enin, A. O., & Ananso, G. N. (2020). Enhanced Biogas Production from Anaerobic Codigestion of Lignocellulosic Waste for Efficient Bioenergy Utilization in Heating and Combustion Engine. Advances in Research, 21(1), 11-21. https://doi.org/10.9734/air/2020/v21i130178
Original Research Article


Nereida C, Naceur B. Chemical composition and pulping of banana pseudo-stems. An International Journal on Industrial Crops and Products. 2004;19(2): 147-157.
DOI: 10.1016/j.indcrop.2003.09.001

Zawawi D, Mohd ZMH, Angzzas SMK, Ashuvila MA. Analysis of the chemical composition and fibre morphology of pineapple (Ananascomosus) leaves in Malaysia. Journal of Applied Sciences. 2014;14(12):1355-1358.
DOI: 10.3923/jas.2014.1358

Kayembe K, Basosila LN, Mpiana PT, Sikulisimwa P, Mbuyu K. Inhibitory effects of phenolic monomers on methanogensis in anaerobic digestion. British Microbiology Research Journal. 2013;3(1):32-41.
DOI: 10.9734/BMRJ/2013/2291

Chen Y, Cheng JJ, Creamer KS. Inhibition of anaerobic digestion process: A review. Bioresource Technology. 2008;99(10): 4044-4064.
DOI: 10.1016/j.biortech.2007.01.057

Eze JL, Ojile O. Anaerobic production from maize wastes. International Journal of Physical Sciences. 2012;7(6):982-987.
DOI: 10.5897/IJPS11.1519

Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtzapple M, Ladisch M. Features of promising technologies for Pre-treatment of lignocellulose biomass. Journal of Bioresource Technology. 2005; 96(6):673–686.

Alvira P, Tomas-Pejo P, Ballesteros M, Negro MJ. Pre-treatment technologies for an efficient bioethanol production based on enzymatic hydrolysis: A review. Bioresource Technology. 2010;101(13): 4851–4861.
DOI: 10.1016/j.biortech.2009.11.093

Yu Q, Liu R, Li K, Ma R. A review of crop straw Pre-treatment methods for biogas production by anaerobic digestion in China. Renewable and Sustainable Energy Reviews. 2019;107:51–58.

Ding T, Hii S, Ong L, Ling T. Investigating the potential of using coconut husk as substrate for bioethanol production. International Conference on Biotechnology and Environmental Management. 2011;18: 29–32.

Mancini G, Papirio S, Lens PNL, Esposito G. Increased biogas production from wheat straw by chemical Pre-treatment, Renewable Energy. 2018;119:608–614.
DOI: 10.1016/j.renene.2017.12.045

Shah TA, Ullah R. Pre-treatment of wheat straw with ligninolytic fungi for increased biogas productivity. International Journal of Environmental Science and Technology. 2019;16:7497-7508.
DOI: 10.1007/s13762-019-02277-8

Harini S, Kumaresan R. Production of cellulase from corn cobs by Aspergillus niger under submerged fermentation. International Journal of Chemical Technology Research. 2014;6(5):2900 ̶ 2904.

Mrudula S, Murugammal R. Production of cellulose by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate. Brazilian Journal of Microbiology. 2011;42:1119-1127.

Eduok S, John O, Ita B, Inyang E, Coulon F. Enhanced biogas production from anaerobic codigestion of lignocellulosic biomass and poultry faeces using source separated human urine as buffering agent. Frontiers in Environmental Science and Environmental Toxicology; 2018.
DOI: 10.3389/fenvs.2018.00067

AOAC. Official methods of analysis of AOAC international. 20th Edition. AOAC International, Gaithersburg, Maryland, USA. 2016;3172.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey's manual of determinative bacteriology, 19th Edn. Williams and Wilkins, MD, USA. 1994;71-84.

Cheesbrough M. District laboratory practices in tropical countries. Cambridge University Press. 2006;62.

Kalia VC, Sonakya V, Raizada N. Anaerobic digestion of banana stems waste. Bioresource Technology. 2000; 73(2):191-193.
DOI: 10.1016/S0960-8524(99)00172-8

Verma D, Gope PC, Shandilya A, Gupta A. Coir fibre reinforcement and application in polymer composites: A review. Journal of Material and Environmental Science. 2013; 4(2):263-276.

Viju N, Satheesh S, Vincent SGP. Antibiofilm activity of coconut (Cocosnucifera Linn.) husk fibre extract. Saudi Journal of Biological Sciences. 2013;20(1):85-91.
DOI: 10.1016/j.sjbs.2012.11.002

Wang Y, Zhang Y, Wang J, Meng L. Effects of volatile fatty acid concentrations on methane yields and methanogenic bacteria. Biomass and Bioenergy. 2009; 33(5):848-853.
DOI: 10.1016/j.biombioe.2009.01.007

Goberna M, Camacho MM, Lopez-Abadia JA, García C. Codigestion, biostimulation and bioaugmentation to enhance methanation of brewer’s spent grain. Waste Management and Research. 2013; 31(8):805–810.

Wang Y, Zang B, Gong X, Liu Y, Li G. Effects of pH buffering agents on the anaerobic hydrolysis acidification stage of kitchen waste. Waste Management. 2017; 68:603–609.

Orhorhoro EK, Ebunilo PO, Sadjere GE. Experimental determination of effect of total solids (TS) and volatile solids (VS) on biogas yield. American Journal of Modern Energy. 2017;3:131-135.
DOI: 10.11648/j.ajme.20170306. 13

Sheng K, Chen X, Pan J. Effect of ammonia and nitrate on biogas production from food waste via anaerobic digestion. Biosystems Engineering. 2013;116(2): 205-212.

Velmurugan R, Alwar RR. Anaerobic digestion of vegetable waste for biogas production in a fed-batch reactor. International Journal of Emerging Science. 2011;1(3):478-486.

Sharma VK, Testa C, Cornacchia G, Lastella G, Farina R. Anaerobic digestion of semi-solid organic waste available from orthofruit market: Preliminary experimental results. Energy Conversion and Management. 1999;40:287-304.

Chaudhari AB, Suryawanshi PC, Kothari RM. Anaerobic digestion: Processes, product and applications. Environmental Science, Engineering and Technology, New York, NY, USA. Nova Science Publishers, Inc; 2012.

de Diego-Díaz B, Fernández-Rodríguez J, Vitas AI, Peñas FJ. Biomethanization of solid wastes from the alcoholic beverage industry: Malt and sloe. Kinetic and microbiological analyses. Chemical Engineering Journal. 2016;33(2):274–315.

Qi G, Pan Z, Sugawa Y, Andriamanohiarisoamanana FJ, Yamashiro T, Iwasaki TM, Kawamoto K, Ihara I, Umetsu K. Comparative fertilizer properties of digestates from mesophilic and thermophilic anaerobic digestion of dairy manure: focusing on plant growth promoting bacteria (PGPB) and environmental risk. Journal of Material Cycles and Waste Management. 2018; 20(3):1448–1457.

Owamah HI, Dahunsi SO, Oranusi US, Alfa MI. Fertilizer and sanitary quality of digestate biofertilizer from the codigestion of food waste and human excreta. Waste Management. 2014;34:747–752.

Alfa MI, Adie DB, Igboro SB, Oranusi US, Dahunsi SO, Akali DM. Assessment of biofertilizer quality and health implications of anaerobic digestion effluent of cow dung and chicken droppings. Renewable Energy. 2014;63:681–686.

European Commission. Commission Regulation (EU) No 142/2011 of 25 February 2011 implementing Regulation (EC) No 1069/2009 of the European Parliament and of the Council laying down health rules as regards animal by-products and derived products not intended for human consumption and implementing Council Directive 97/78/EC as regards certain samples and items exempt from veterinary checks at the border under that Directive, Official Journal of the European Union. 2011; 54.

Liu X, Lendormi TA, Lanoisellé J. A review of hygienization technology of biowastes for anaerobic digestion: Effect on pathogen inactivation and methane production. Chemical Engineering Transactions. 2018; 70.
DOI: 10.3303/CET1870089